chicken antibody against vap (Bethyl)

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Bethyl chicken antibody against vap

CK1G3 localizes to cytoplasmic punctate compartments Intracellular localization of HA-CK1G3. CK1G3 KO cells expressing HA-CK1G3 were immunostained with HA antibody and antibodies against various organelle markers: LBPA (late endosomes), EEA1 (early endosomes), Lamp2 (lysosomes), Hrs (exosomes), <t>VAP</t> <t>(ER),</t> <t>TGN46</t> (TGN), Rab11a (recycling endosomes and Rab11a-positive secretory vesicles), catalase (peroxisomes), TOMM20 (mitochondria), caveolin (caveolae), and lipid dye II (lipid droplet). For the visualization of lipid droplets, cells were pre-incubated in a medium containing 100 μM oleic acid for 24 h before fixation. The scale bars indicated in microscopy images represent 10 μm.

Chicken Antibody Against Vap, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chicken+antibody+against+vap/TGOLN2+Antibody/pmc09254030-256-12-21
Average 93 stars, based on 3 article reviews

chicken antibody against vap - by Bioz Stars, 2026-09

93/100 stars

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1) Product Images from "Compartmentalization of casein kinase 1 γ CSNK1G controls the intracellular trafficking of ceramide"

Article Title: Compartmentalization of casein kinase 1 γ CSNK1G controls the intracellular trafficking of ceramide

Journal: iScience

doi: 10.1016/j.isci.2022.104624

Figure Legend Snippet: CK1G3 localizes to cytoplasmic punctate compartments Intracellular localization of HA-CK1G3. CK1G3 KO cells expressing HA-CK1G3 were immunostained with HA antibody and antibodies against various organelle markers: LBPA (late endosomes), EEA1 (early endosomes), Lamp2 (lysosomes), Hrs (exosomes), VAP (ER), TGN46 (TGN), Rab11a (recycling endosomes and Rab11a-positive secretory vesicles), catalase (peroxisomes), TOMM20 (mitochondria), caveolin (caveolae), and lipid dye II (lipid droplet). For the visualization of lipid droplets, cells were pre-incubated in a medium containing 100 μM oleic acid for 24 h before fixation. The scale bars indicated in microscopy images represent 10 μm.

Techniques Used: Expressing, Incubation, Microscopy

Compartmentalization-dependent functional control of CK1Gs After newly synthesized as a cytosolic protein, CK1G presumably binds to the Golgi-membrane via the electrostatic interaction between its C -terminal basic amino acids stretch and the Golgi localizing PtdIns(4)P (see also the text) and subsequently palmitoylated by the Golgi-residing palmitoyl acyltransferase (PAT). Palmitoylated CK1G is largely distributed to post-Golgi compartments and only partly to the Golgi apparatus, where SM synthase 1 (SMS1) is localized. CERT is associated with the ER via VAP-binding and with the Golgi via PtdIns(4)P-binding. It remains elusive whether palmitoylated CK1Gs are recycled among the distal- and post-Golgi compartments or eventually directed to lysosomes (for degradation) or exosomes (for secretion). The Golgi-distributed CK1G is spatially limited to interact only with CERT recruited to the ER-Golgi contact zone. In contrast, the C -terminus deleted CK1Gs are distributed throughout the cytosol and can phosphorylate CERT anywhere in the cells once the priming phosphorylation of CERT S132 by PKD occurs.

Figure Legend Snippet: Compartmentalization-dependent functional control of CK1Gs After newly synthesized as a cytosolic protein, CK1G presumably binds to the Golgi-membrane via the electrostatic interaction between its C -terminal basic amino acids stretch and the Golgi localizing PtdIns(4)P (see also the text) and subsequently palmitoylated by the Golgi-residing palmitoyl acyltransferase (PAT). Palmitoylated CK1G is largely distributed to post-Golgi compartments and only partly to the Golgi apparatus, where SM synthase 1 (SMS1) is localized. CERT is associated with the ER via VAP-binding and with the Golgi via PtdIns(4)P-binding. It remains elusive whether palmitoylated CK1Gs are recycled among the distal- and post-Golgi compartments or eventually directed to lysosomes (for degradation) or exosomes (for secretion). The Golgi-distributed CK1G is spatially limited to interact only with CERT recruited to the ER-Golgi contact zone. In contrast, the C -terminus deleted CK1Gs are distributed throughout the cytosol and can phosphorylate CERT anywhere in the cells once the priming phosphorylation of CERT S132 by PKD occurs.

Techniques Used: Functional Assay, Control, Synthesized, Membrane, Binding Assay, Phospho-proteomics

Figure Legend Snippet:

Techniques Used: Transduction, Recombinant, Protease Inhibitor, Software

Expressing:

Article Title: Compartmentalization of casein kinase 1 γ CSNK1G controls the intracellular trafficking of ceramide
Article Snippet: Anti-HA antibody (#3F10) was purchased from Roche Diagnostics; anti-CERT (#ab72536) from Abcam; chicken antibody against VAP ( ) anti-TGOLN2/TGN46 (#A304-434A) from Bethyl Laboratories; anti-TOMM20 (#WH0009804M1) from Sigma-Aldrich; anti-Lamp2 (#sc-18822) from Santa Cruz; anti-LBPA (#z-PLBPA) from Echelon Bioscience; anti-EEA1 (#610457) from BD Transduction Laboratories; anti-catalase (#D4P7B) and anti-Rab11a (#2413) from Cell Signaling Technology; anti-Hrs (#10390-1-AP) from Proteintech; anti-GFP (#04404-84) from Nacalai Tesque, and anti-GAPDH (016-25523) from Fujifilm Wako Pure Chemical Corporation.

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